发明名称 |
Avian pluripotent embryonic germ cell line |
摘要 |
Disclosed in this invention is a process for preparing an established avian embryonic germ cell line comprising the steps of: (a) culturing primordial germ cells (PGCs) isolated from an avian embryonic gonad in a medium supplemented with a cell growth factor and a differentiation inhibitory factor to obtain EG cell colonies; (b) culturing the EG cells in the same medium as in step (a) by employing a feeder layer until the EG cells are colonized; and (c) recovering and subculturing the EG cells in the same medium as in step (a) to establish the EG cell line. |
申请公布号 |
AU768357(B2) |
申请公布日期 |
2003.12.11 |
申请号 |
AU20000025780 |
申请日期 |
2000.02.11 |
申请人 |
HANMI PHARM. CO., LTD.;JAE YONG HAN |
发明人 |
JAE YONG HAN;TAE SUB PARK |
分类号 |
C12N15/09;C12N5/073;C12N5/10;C12Q1/02;(IPC1-7):C12N5/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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