METHOD OF CONSTRUCTING cDNA TAG FOR IDENTIFYING EXPRESSED GENE AND METHOD OF ANALYZING GENE EXPRESSION

摘要

There are provided a method for the preparation of cDNA tags for identifying expressed genes and a method for analysis of gene expression. The cDNA tags for identifying expressed genes are prepared by the method comprising a kind of type II restriction enzyme, two kinds of type IIS restriction enzymes and linkers X and Y having a recognition site for one of two kinds of type IIS restriction enzymes. The cDNA tags can be used alone or in combination like chain (concatemer) formed by combining process to analyze gene expression.