| 摘要 |
Determining the degree of methylation of a target cytosine in the genome of a cell or organism comprises treating a DNA sample with bisulfite, amplifying a fragment of the DNA, sequencing the amplified fragment to generate an electropherogram and determining the rate of conversion of cytosine to uracil taking into account the relation of the signal intensity of bases whose hybridization behavior is altered by bisulfite treatment to that of bases whose hybridization behavior is not so altered. Determining the degree of methylation of a target cytosine in the genome of a cell or organism comprises treating a DNA sample with bisulfite, amplifying a fragment of the DNA, sequencing the amplified fragment to generate an electropherogram, determining the rate of conversion of cytosine to uracil as a result of bisulfite treatment taking into account the relation of the signal intensity of bases whose hybridization behavior is altered by bisulfite treatment to the signal intensity of bases whose hybridization behavior is not altered by bisulfite treatment, with reference to the sense sequence and/or its reverse complementary sequence, and calculating the degree of methylation from the determined conversion rate.
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