PROCESS FOR THE ISOLATION AND PURIFICATION OF A GLYCOPROTEIN AVIDIN
摘要
<p>The present invention provides a process for the preparation of Avidin having a high purity of 98 % from egg white, the said process comprising lyophilizing the homogenized egg white with buffer followed by equilibrating the lyophilized material with cation exchanger for an hour at a temperature range of 20 DEG to 30 DEG C; filtering the mixture to obtain a filtrate and matrix residue; washing the matrix twice with buffer; separating the washings to obtain washed matrix; adding 0.01 M dye in buffer to the washed matrix; equilibrated for an hour at an ambient temperature; separating the supernatant; adding acetic acid to supernatant and dialysing the solution till it is decolorised to obtain pure Avidin.</p>
申请公布号
WO03099035(A1)
申请公布日期
2003.12.04
申请号
WO2002IB03044
申请日期
2002.08.02
申请人
INDIAN INSTITUTE OF TECHNOLOGY;RAO, MUKKAVILLI, VENKATA, SUBBA;GUPTA, MUNISHWR, NATH;ROY, IPSITA