ASSAYS FOR MODULATORS OF ASPARAGINYL HYDROXYLASE

摘要

A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase an d a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absen ce of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-l.alpha., a fragment thereof comprising Asn 803 of HIF-l.alpha. or a peptide analogue of HIF-l.alpha. or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-l.alpha. and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.