LIVING CELL COUNTING METHOD AND DEVICE

摘要

A living cell counting method and device for counting living cells such as microbe in a sample or living cells of a cell tissue is labeled by a fluorescent reagent. Before living cells are fluorescence-labeled, a fluorescence image (first image) of a sample is captured. After the living cells are fluorescence labeled, a fluorescence image (second image) of the sample is captured. The difference (B-A) in number of bright points between the first and second images is determined. In another method, a difference image between the first and second images is created, and the number of bright points in the difference image is counted, or the number of bring points, out of the bright points in the second image, not in the blind area around each bright point in the first image is counted. The influence of fluorescent impurities can be eliminated irrespective of the properties of the sample, and thus the measurement accuracy is improved.