The invention disclose a method for purifying microbial protease, comprising:(i) providing an aqueous liquid sample containing a microbial protease, and a separation medium comprising a base matrix and a plurality of attached ligands that are capable of binding to microbial protease;(ii) contacting separation medium with the sample under conditions permitting binding of microbial protease to the separation medium; and (iii) desorbing microbial protease from the separation medium,wherein the base matrix is hydrophilic and the plurality of ligands are hydrocarbon groups in which all carbon atoms are sp3-hybridised.
申请公布号
WO03100048(A1)
申请公布日期
2003.12.04
申请号
WO2003EP05724
申请日期
2003.05.28
申请人
DSM IP ASSETS B.V.;BECKHOVEN, VAN, RUDOLF, FRANCISCUS, WILHELMUS, CORNELIS;NAEYE, THIERRY, JEAN-BERNARD
发明人
BECKHOVEN, VAN, RUDOLF, FRANCISCUS, WILHELMUS, CORNELIS;NAEYE, THIERRY, JEAN-BERNARD