| 摘要 |
The invention concerns new oxazine derivatives of the general formula I in which R1, R4, R5, R6, R7, R10 denote hydrogen, alkyl, hydroxy, halogen, carboxyl, sulfonyl or amino and R2, R3 denote hydrogen, alkyl, alkoxy, polyoxyhydrocarbyl units, phenyl, phenylalkyl which can be substituted by hydroxy, sulfonyl, carboxy, amino, alkoxycarbonyl, in which R2 with R1 or R3 with R4 can form a saturated or unsaturated C2 or C3 bridge or R2 with R3 can form a saturated or unsaturated C4 or C5 bridge and R8, R9 denote hydrogen, alkyl, alkoxy, polyoxyhydrocarbyl units, phenyl, phenylalkyl which can be substituted by hydroxy, sulfonyl, carboxy, amino, alkoxy-carbonyl in which R8 with R7 or R9 with R10 can form a saturated or unsaturated C2 or C3 bridge or R8 with R9 can form a saturated or unsaturated C4 or C5 bridge and in which at least one of the residues R2, R3, R8 or R9 represents a non-bridge forming residue which is additionally substituted by an activated group capable of coupling or by a group that can be activated to couple. and in which at least one of the residues R2, R3, R8 or R9 represents a bridge-forming residue which can be optionally substituted by alkyl. The derivatives serve to produce fluorescent conjugates for use in immunoassays and for DNA analysis.
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