发明名称 |
Method and apparatus for recognizing molecular compounds |
摘要 |
A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass-enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.
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申请公布号 |
US2003224370(A1) |
申请公布日期 |
2003.12.04 |
申请号 |
US20020158995 |
申请日期 |
2002.05.31 |
申请人 |
RASSMAN WILLIAM;RALIN DAVID;ZHOU FEIMING;HAN SHUBO |
发明人 |
RASSMAN WILLIAM;RALIN DAVID;ZHOU FEIMING;HAN SHUBO |
分类号 |
C12Q1/68;C40B70/00;G01N21/55;G01N33/53;(IPC1-7):C12Q1/68;G01J3/45;G01B9/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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