| 摘要 |
1,201,747. Imidazofe-glycerol by fermentation. KYOWA HAKKO KOGYO K.K. 28 March, 1969 [29 March, 1968], No. 16489/69. Heading C2C. Imidazole-glycerol (4-(1<SP>1</SP>,2<SP>1</SP>,3<SP>1</SP>-trihydroxypropyl)-imidazole) is produced by culturing a histidine-requiring strain of an organism of the genus Brevibacterium or Corynebacterium. The preferred organisms are B. ammoniagenes ATCC 21225, and C. glutamisum ATCC 21224, and the process is normally carried out at pH 6.0-8.5, and between 23‹ C. and 40‹ C. for 3 to 7 days. Specified carbon sources are carbohydrates, viz glucose, fructose, galactose, sucrose, maltose, trehalose, cellobiose and arabinose; nitrogen sources include ammonia, urea and ammonium salts, and the medium also contains inorganic salts, trace amounts of biotin, thiamine, #-alumine, nicotinic acid, and/or purine bases, and histidine (or a histidine containing material) in an amount of from 50-300 y/ml. of the medium. The yield is enhanced by the presence of phosphate ion (up to 0.05 mol./l.) and the imidazole-glycerol can be recovered by precipitation as the mercury salt in addition to the usual procedures.
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