| 摘要 |
The present invention relates to a method for production of a protein having a desired fold. This is especially achieved by subjecting a population of proteins to a separation step under non-reducing conditions. This allows for identification of a sub-population of proteins having the disulfide bond configuration resulting in a desired fold. Most often this will be the protein of proper structure and/or function. Thus, by using the novel method the purity of the protein having a desired fold can be increased as compared to the purity of a similar protein produced by a conventional method. Important aspect of the invention is a functional active MHC heavy chain protein obtainable by the above method and the use of a MHC heavy chain protein in analysis of peptide binding capacity. |
