AN ELISA MEASURING DE NOVO PEPTIDE-MHC INTERACTION

摘要

The present invention relates to a quantitative method to measure the interaction between a peptide and a MHC molecule. The basis for measuring this interaction is substantially pure denatured MHC heavy chains. These denatured MHC heavy chains are subsequently renatured in a buffer containing excess of beta2m and an increasing concentration of the peptide in question, leading to a de novo folding and generation of peptide-MHC complexes. The present invention is exemplified with reference to MHC class I proteins, but it is envisaged that it is possible on a similar manner to generate data from any type of complex where de novo folding is peptide dependent, e.g., MHC class II.