| 摘要 |
Restriction Independent Cloning Events (RICE) are made by generating 5' overhangs (sticky ends). The polynucleotides to be joined are reacted with a DNA polymerase, having 3' to 5' exonuclease activity and 5' to 3' polymerizing activity, less than all of the dNTPs, a kinase (optional) and a ligase. The complementary 5' overhangs anneal and ligate.
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