| 摘要 |
A method for determining the level of kinase activity or phosphatase activit y in a sample without the use of antibodies or radioactive labels is disclosed . The method employs a fluorescently-labeled phosphorylatable reporter peptide that is capable of being cleaved by a protease only when the peptide is in a n unphosphorylated state. A change in fluorescence characteristics is an indication that the peptide is cleaved and, therefore, in an unphosphorylate d state. Thus, the level of protease cleavage, as measured by the fluorescence change, provides a direct measure of phosphatase activity whereas the level of kinase activity is inversely proportion to the level of protease cleavage. T he method is particularly well suited to high throughput screening, for example , for screening compounds which modulate kinase or phosphatase activity.</SDOA B>
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