发明名称 |
METHOD FOR NON-HOMOLOGOUS DNA RECOMBINATION PROCESS IN MAMMALIAN CELLS |
摘要 |
The invention concerns an isolated hybrid double-strand DNA molecule for use as non-homologous recombination substrate comprising: a single unidirectional promoter and at least two different reporter genes, respectively A and C in the direction of transcription; the only gene under the control of said promoter being the reporter gene A, and two identical sites of directed double-strand cleavage, the first cleavage site being located between the promoter and the reporter gene A and the second cleavage site being located upstream of the reporter gene C on therein and the use of said hybrid double-strand DNA molecule for measuring the non-homologous DNA recombination or non-homologous end joining in mammalian cells, as well as for screening anti-cancer and anti-retroviral drugs.
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申请公布号 |
WO03097842(A1) |
申请公布日期 |
2003.11.27 |
申请号 |
WO2003FR01486 |
申请日期 |
2003.05.15 |
申请人 |
COMMISSARIAT A L'ENERGIE ATOMIQUE;INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;HUCK, SYLVIE;LOPEZ, BERNARD;BARBAT, JOSEE |
发明人 |
HUCK, SYLVIE;LOPEZ, BERNARD;BARBAT, JOSEE |
分类号 |
C07K14/705;C07K14/73;C07K14/74;C12N15/12;C12N15/85;(IPC1-7):C12N15/85;A01K67/027;C12N5/10;C12Q1/68 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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