发明名称 |
METHOD OF EFFECTING LYSIS OF ACID-FAST BACTERIA AND METHOD OF PERFORMING GENE AMPLIFICATION OR DETECTION THEREWITH |
摘要 |
A method of effecting lysis of acid-fast bacteria, comprising heating acid-fast bacteria in a liquid containing a nonionic surfactant at a temperature of below the boiling point of the liquid. This method enables accomplishing secure lysis of acid-fast bacteria in a simple manner within a short period of time without the use of special apparatus and agent and enables extracting genes. The heating is preferably conducted at 96ºC for 10 min. As the nonionic surfactant, use can be made of a d-sorbitol fatty acid ester, a polyoxyethylene glycol sorbitan alkyl ester, a polyoxyethylene glycol p-t-octylphenyl ether or the like. The pH value of the liquid is preferably 8, and the liquid preferably contains EDTA. It is also preferred that before the heating, the acid-fast bacteria be treated with lipase.
|
申请公布号 |
WO03097816(A1) |
申请公布日期 |
2003.11.27 |
申请号 |
WO2003JP06321 |
申请日期 |
2003.05.21 |
申请人 |
ARKRAY, INC.;KAMATA, TATSUO;IZUMIZAWA, YUJI |
发明人 |
KAMATA, TATSUO;IZUMIZAWA, YUJI |
分类号 |
C12N1/06;C12N15/10;C12Q1/68;(IPC1-7):C12N1/06 |
主分类号 |
C12N1/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|