摘要 |
Method and reagent kit for the detection of a protease activated in apoptosis or of apoptotic cells in a biological sample comprising the following steps: contacting the sample with (a) a binding partner which specifically binds a protease activated in apoptosis but does not block the active centre and is bound or can be bound to a solid phase, (b) a substrate that is specific for the protease and (c) a reaction buffer, and determining the chromogen or fluorochrome formed in the reaction solution as a measure for the apoptotic cells and/or activated protease contained in the sample.
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