DETECTION OF CLONAL T-CELL RECEPTOR-GAMMA GENE REARRANGEMENT BY PCR/TEMPORAL TEMPERATURE GRADIENT GEL ELECTROPHORESIS (TIGE)

摘要

The present invention provides methods and compositions for detecting and analyzing clonal T-cell receptor (TCR) gene rearrangement using temporal temperature gradient gel electrophoresis (TTGE), which employs a gradual and uniform increase in the temperature of a constant denaturing gel to resolve different DNA molecules based on base pair composition. The present invention also provides methods and compositions for providing appropriate DNA migration markers for TTGE analysis.