发明名称 |
Method for producing L-carnitine from crotonobetaine using a two stage continuous cell-recycle reactor |
摘要 |
L(-)-carnitine is synthesized from crotonobetaine, crotonobetaine salts or derivatives in an ecologically advantageous manner by immobilizing cells of Escherichia coli 044 K74 on ceramics, glass beads or polyurethane disks in a two stage continuously operating cell recycle reactor containing a reaction medium. The medium preferably contains between 25 mM and 1 M crotonobetaine and at least 50 mM fumarate. Growing or resting cells of E. coli are retained in the reactor by micro or ultrafiltration membranes which are arranged as a flat membrane module or hollow fiber module. A first stage contains a reactor tank and a second stage contains an external recirculation loop connected to the tank for feeding the reaction medium through a filter unit. L-carnitine is synthesized under anaerobic conditions to produce a reaction medium containing L-carnitine and unreacted crotonobetaine. The reaction medium is transferred through the recirculation loop to the filter unit to produce a filtrate containing L-carnitine and a residue containing unreacted crotonobetaine and cells. The residue is recirculated to the reactor tank.
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申请公布号 |
US6653112(B2) |
申请公布日期 |
2003.11.25 |
申请号 |
US20000530783 |
申请日期 |
2000.05.12 |
申请人 |
SIGMA-TAU INDUSTRIE FARMACEUTICHE RIUNITE S.P.A. |
发明人 |
KLEBER HANS-PETER;CANOVAS-DIAZ MANUEL;OBON JOSE MARIA;IBORRA JOSE MARIA |
分类号 |
C12P1/00;C12P13/00;C12R1/19;(IPC1-7):C12P13/00;C12P7/40;C12N11/14;C12N11/08;C12N1/20 |
主分类号 |
C12P1/00 |
代理机构 |
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