BIOSENSOR FOR DETECTION OF PHENOL COMPOUNDS AND PREPARATION METHOD THEREOF

摘要

PURPOSE: A biosensor for detection of phenol compounds and a preparation method thereof are provided, thereby rapidly detecting phenol compounds without pretreatment regardless of time and place. CONSTITUTION: A biosensor for detection of phenol compounds comprises a first nucleic acid molecule containing the nucleotide sequence encoding a reporter molecule; a gene construct containing a phenol compound degrading enzyme regulating gene capR, a promoter gene Pr regulating the expression of the enzyme regulating gene capR and a second nucleic acid molecule with a promoter gene Po regulating the synthesis of the phenol compound degrading enzyme; and a cell containing the gene construct, wherein the first nucleic acid molecule encodes a firefly luciferase gene luc; the biosensor comprises a detection unit of the activity of luciferase when the cell is exposed to phenol; the second nucleic acid molecule is derived from Pseudomonas putida KCTC 1452; and the cell is a freeze-dried powder form.