Protein purification and detection methods

摘要

The present invention relates to methods for purifying and for detecting the presence of a protein. The invention employs a NorpA sequence and a PDZ1 domain. A protein tagged with a NorpA sequence can associate with PDZ1 domain. Similarly, a protein tagged with a PDZ1 domain can associate with a NorpA sequence. This interaction forms an aspect of the protein purification methods and protein detection methods of the present invention. Recombinant expression vectors and a protein purification solid phase are also disclosed, as well as protein detection and purification kits.