发明名称 |
STRAIN OF BACTERIUM SALMONELLA TYPHIMURIUM = 14 USED FOR PREPARING VACCINES AND DIAGNOSTIC PREPARATIONS |
摘要 |
FIELD: veterinary microbiology, vaccines. SUBSTANCE: strain of bacterium Salmonella typhimurium = 14 shows morphological, cultural and enzymatic properties that are typical for salmonella genus. The proposed strain by its serological properties is agglutinated with salmonellous monoreceptor sera 04 (1 and 2 complex) H-1. Strain is a producer of endotoxin and hemolysine. Strain shows protein structure, its nucleotide DNA composition (% GC) is 53.1, it comprises plasmids of molecular mass 64 mDa and it is lysed with salmonellous bacteriophage. Virulence LD50 for white mice is 1.7 x 106 live bacterial cells in intraperitoneal administration. Strain is pathogenic for calves and profuse diarrhea is noticed on 2-d day and animal death on 3-4-th day. Concentration of bacterial cells in the strain culturing is 40-50 billion cells by optical turbidity standard GISK and protective effect after infection with virulent strains occurs on 5-7-th day. The strain is useful for preparing vaccines against salmonellosis in animals and for preparing diagnostic preparation and preparing hyperimmunic curative sera and globulins also. EFFECT: valuable medicinal properties of strain and vaccine. |
申请公布号 |
RU2216350(C2) |
申请公布日期 |
2003.11.20 |
申请号 |
RU20020100765 |
申请日期 |
2002.01.18 |
申请人 |
MOSKOVSKAJA GOSUDARSTVENNAJA AKADEMIJA VETERINARNO;MEDITSINY I BIOTEKHNOLOGII IM |
发明人 |
VORONIN E.S.;DEVRISHOV D.A.;BEDOEVA Z.M.;PIMENOV E.V.;KUZNETSOV S.M.;SHVEDOV V.V. |
分类号 |
A61K39/112;C12N1/20;C12R1/42 |
主分类号 |
A61K39/112 |
代理机构 |
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