| 摘要 |
Three-dimensional (3D) reconstruction of a cell (1) includes adjusting a current set of projection images (8, 9) according to a priori knowledge (24) to produce adjusted projection images (9), for example, based on probability masks and/or Bayesian analysis (16) of multiple similar objects in the same sample (30). A reconstruction algorithm processes the adjusted projection images to generate a 3D image (10). The 3D image is further adjusted accordi ng to the a priori knowledge (24) to generate an adjusted 3D image (11). Criter ia for process completion are applied to determine whether the adjusted 3D imag e is adequate (12). Otherwise, a set of pseudo projections are computationally created at the same projection angles as the current set of projection image s (8, 13) and then compared to the current set of projection images (8) to produce a set of new projections (14), wherein the new projections are input again to the reconstruction algorithm and the steps of the method are repeat ed until the adequacy criteria are met (15). |
