SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS

摘要

A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.