EXPRESSION CONSTRUCTS FOR PRODUCING DOUBLE-STRANDED (DS) RNA AND THE USE THEREOF

摘要

The invention relates to a polynucleotide containing an inner polynucleotide, which is functionally linked to a first eukaryotic expression control sequence at the 5' end and which is functionally linked to a second eukaryotic expression control sequence at the 3' end, whereby: (i) only the first eukaryotic expression control sequence is functionally linked to a first polyadenylation sequence at the 5' end, and the polyadenylation sequence is functional in the 3' to 5' orientation or; (ii) only the second eukaryotic expression control sequence is functionally linked to a second polyadenylation sequence at the 3' end, and the polyadenylation sequence is functional in the 5' to 3' orientation or; (iii) the first eukaryotic expression control sequence is functionally linked to a first polyadenylation sequence at the 5' end and the polyadenylation sequence is functional in the 3' to 5' orientation, and the second eukaryotic expression control sequence is functionally linked to a second polyadenylation sequence at the 3' end and the polyadenylation sequence is functional in the 5' to 3' orientation. The invention also relates to methods for producing double-stranded polynucleotides. The invention additionally concerns vectors and mixtures of vectors, to methods for producing vectors that comprise the inventive polynucleotides or comprise the polynucleotides produced according to the inventive methods, and to host cells containing these vectors. Additionally disclosed are methods for identifying genes whose inactivation leads to detectable changes in a target cell. The invention additionally relates to transgenic animals containing an inventive polynucleotide. Finally, the invention discloses the use of the inventive polynucleotide for producing a medicament for treating and preventing diseases.