The invention relates to the use of an influenza antigen preparation obtainable by the following process, in the manufacture of an intradermal flu vaccine: (i) harvesting of virus-containing material from a culture; (ii) clarification of the harvested material to remove non-virus material; (iii) concentration of the harvested virus; (iv) a further step to separate whole virus from non-virus material; (v) splitting of the whole virus using a suitable splitting agent in a density gradient centrifugation step; (vi) filtration to remove undesired materials; wherein the steps are performed in that order but not necessarily consecutively.
申请公布号
EP1361889(A1)
申请公布日期
2003.11.19
申请号
EP20020722113
申请日期
2002.02.21
申请人
GLAXOSMITHKLINE BIOLOGICALS S.A.
发明人
GARCON, NATHALIE;SLAOUI, MONCEF, MOHAMED;VAN HOECKE, CHRISTIAN