| 摘要 |
The invention provides a mouse model of hepatocellular carcinoma and a method for establishing an animal model comprising: a) inserting an exogenous gene of interest into a suitable vector carrying sequences homologous to a target site, to construct a recombinant targeting vector; b) transfecting ES cells of a studied animal using said targeting vector from step a), and screening targeted ES cells which have integrated the exogenous gene into a specific site; c) injecting targeted ES cells obtained from step b) into said animal's blastulas and culturing the blastulas in vitro, so that embryos containing targeted ES cells are generated; and d) implanting embryos generated from step c) into said animal's uterus, thereby progeny expressing the exogenous gene stably is developed. <??>The invention further relates to the use of said transgenic mouse in research of hepatocellular carcinoma pathogenesis, its early stage diagnosis and therapy, and screening of anticancer drugs. <IMAGE>The invention provides a mouse model of hepatocellular carcinoma and a method for establishing an animal model comprising: a) inserting an exogenous gene of interest into a suitable vector carrying sequences homologous to a target site, to construct a recombinant targeting vector; b) transfecting ES cells of a studied animal using said targeting vector from step a), and screening targeted ES cells which have integrated the exogenous gene into a specific site; c) injecting targeted ES cells obtained from step b) into said animal's blastulas and culturing the blastulas in vitro, so that embryos containing targeted ES cells are generated; and d) implanting embryos generated from step c) into said animal's uterus, thereby progeny expressing the exogenous gene stably is developed. <??>The invention further relates to the use of said transgenic mouse in research of hepatocellular carcinoma pathogenesis, its early stage diagnosis and therapy, and screening of anticancer drugs. <IMAGE>
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