MEANS AND METHODS FOR DETECTING ENDOGLYCOSIDASE ACTIVITY

摘要

The invention relates to detection of endoglycosidase activity and diagnostics of infertility and diseases wherein an altered amount of an endoglycosidase is involved. Two methods for detecting the endoglycosidase activity are disclosed that solve the problem of interference of the additional transglycosidase activity of endoglycosidases in an assay for endoglycosidase activity at high substrate concentrations. The first method involves substrates that cannot be glycosylated due to the absence of a hydroxyl group at the 4 position of the reducing end sugar substrate. The second method involves a further other molecule capable of being glycosylated for competition with the transglycosylation of the substrate. Methods of the synthesis of glycosylated substrates using the transglycosidase activity of endoglycosidases are also claimed.