发明名称 |
EXPRESSION SYSTEM FOR LARGE FUNCTIONAL PROTEINS |
摘要 |
The present invention provides a system and method for expressing functional ABC (ATP-binding cassette) proteins, from the ABCA subfamily, in a host cell . A system comprises two or more expression vectors each comprising a nucleic acid molecule encoding one or more domains of an ABC transporter gene and a means for expressing the nucleic acid molecule. Each expression vector of th e system includes a nucleic acid molecule that encodes a domain that is functionally complementary to domains contained in the other expression vectors of the system but when taken together comprise the full ABC transporter gene. Co-transfection of the expression vectors into a host cell provides co-expression of each of the domains of the protein which associate to form an ABC transporter protein having functional characteristics of the full-length protein.
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申请公布号 |
CA2485110(A1) |
申请公布日期 |
2003.11.13 |
申请号 |
CA20032485110 |
申请日期 |
2003.05.06 |
申请人 |
UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
HAUSWIRTH, WILLIAM S.;AHN, JINHI;MOLDAY, ROBERT S. |
分类号 |
A61K31/7088;A61K48/00;C12N15/12;C12N15/63;(IPC1-7):C12N15/12;A61K31/708 |
主分类号 |
A61K31/7088 |
代理机构 |
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