| 摘要 |
<p>The invention relates to the inhibition of viral gene expression. More specifically, the invention relates to a multimeric self-cleaving ribozyme construct, which includes a plurality of operably linked self-cleaving monomeric units wherein said monomeric units include at least one first monomeric unit and at least one second monomeric unit. The first and second monomeric units include a first ribozyme and a second ribozyme respectively having trans-cleavage specificity, with the ribozymes functioning both in cis and in trans and having catalytic domains and antisense domains. The ribozymes have cis target recognition sequences including ribozyme cleavage sites recognizable by the antisense domains, wherein the cis target recognition sequences are the same as or similar to target recognition sequences of a target transcript or a portion of a target transcript sequence which retains said ribozyme cleavage sites and which are recognizable by said antisense domains.</p> |
