Use of an anion exchange resin (emp-7) as solid support for peptide synthesis and affinity chromatography

摘要

The present invention refers to the discovery that the anion exchange resin DEAE-Sephadex A50(TM) might be also used alternatively as polymeric support for peptide synthesis and affinity chromatography. It was possible to synthesize a model octapeptide (angiotensin II) in this resin using the Fmoc-chemistry and when appropriate "linker groups" are used, free peptide cleaved from the resin can be obtained containing C-terminal extremity in carboxylate or carboxamide forms. By considering the feasibility of synthesizing peptide sequence throughout its structure, the repetitive (NANP)3 sequence found in the antigenic epitope of the sporozoite form of Plasmodium falciparum involved in the malaria transmission was assembled in the DEAE-Sephadex A50 resin. Column with the synthesized (NANP)3-containing DEAE-Sephadex A50 resin retained quantitatively antibody for (NANP)3 sequence which was further removed through usual washing process, thus demonstrating the feasibility of using this anion.