Insulator element having enhancer-blocking properties

摘要

The present invention relates to nucleic acid sequences that have insulator activity and the use of such sequences to prevent position effects on gene constructs transfected into cells. In one embodiment as shown in FIGS. 5A and 5B a vector is provided with a polylinker site (a) for insertion of a gene, wherein the polylinker site is flanked by one or more insulators (b). This insulator blocks enhancer effects on genes located downstream from the enhancer when the insulator is positioned between the enhancer and the gene.