| 摘要 |
The present invention provides voltage-gated K<+> channels with amino acid substitutions that produce a constitutively open phenotype when expressed in the cell. The invention includes mutated purified proteins with constitutively open voltage-gated K<+> channel activity, for example those derived from Shaker, Shab, Shal, and Shaw family of voltage-gated K<+> channels. The invention also includes nucleic acid encoding constitutively open voltage-gated K<+> channels, as well as cells and transgenic animals expressing constitutively open voltage-gated K<+> channels. Methods are provided for screening substances for ability to modulate voltage-gated K<+> channels, by exploiting the ability of constitutively active voltage-gated K<+> channels to affect growth or other phenotypic or genotypic characteristics in eukaryotic cells.
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