| 摘要 |
A novel technique is provided, designated DamID, for the identification of DNA loci that interact in vivo with specific nuclear proteins in eukaryotes. By tethering a DNA modification enzyme, in particular, E. coli DNA adenine methyl transferase (Dam), to a chromatin protein. The DNA modification enzyme (Dam) can be targeted in vivo to the native binding loci of the protein, resulting in local DNA modification. Sites of DNA modification can subsequently be mapped using modification-specific restriction enzymes, antibodies, or DNA array methods. DNA Modification Identification (DamID) has potential for genome-wide mapping of in vivo target binding sites of chromatin proteins in various eukaryotes.
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