发明名称 |
A METHOD OF PREPARING CROSS-LINKED ENZYME AGGREGATES |
摘要 |
The invention relates to a method of preparing cross-linked enzyme aggregates (CLEAs) comprising the steps of a) precipitating a dissolved enzyme present in a liquid medium: b) cross-linking with a cross-linker, and c) optionally, washing the cross-linked enzyme aggregate. According to the invention at least one treatment is performed chosen from the group consisting of I) performing step a) in the presence of a compound selected from the group consisting of i) a crown ether, and ii) a surfactant, and II) performing step c) with a second liquid differing in organic composition from the liquid. |
申请公布号 |
EP1360285(A1) |
申请公布日期 |
2003.11.12 |
申请号 |
EP20020711535 |
申请日期 |
2002.02.01 |
申请人 |
TECHNISCHE UNIVERSITEIT DELFT |
发明人 |
LOPEZ SERRANO, PALOMA;CAO, LINQIU;VAN RANTWIJK, FREDERIK;SHELDON, ROGER, ARTHUR |
分类号 |
C12N11/06;C12N9/20;C12N9/96;C12N11/02;C12P7/00 |
主分类号 |
C12N11/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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