发明名称 |
Fremgangsmåter og blandinger for analysering av proteiner |
摘要 |
Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags. |
申请公布号 |
NO20035010(A) |
申请公布日期 |
2003.11.11 |
申请号 |
NO20030005010 |
申请日期 |
2003.11.11 |
申请人 |
ACLARA BIOSCIENCES INC |
发明人 |
SALIMI-MOOSAVI, HOSSEIN;SINGH, SHARAT;TAHIR, SYED HASAN;WALLWEBER, GERALD J;KIRAKOSSIAN, HRAIR;MATRAY, TRACY J;HERNANDEZ, VINCENT S |
分类号 |
G01N33/483;G01N21/78;G01N27/447;G01N33/53;G01N33/536;G01N33/68 |
主分类号 |
G01N33/483 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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