发明名称 |
SEMISYNTHETIC PROTEIN-BASED SITE-DIRECTED PROBES FOR IDENTIFICATION AND INHIBITION OF ACTIVE SITES, AND METHODS THEREFOR |
摘要 |
A C-terminally modified Ubiquitin (Ub) derivative, ubiquitin vinyl sulfone (UbVS), which is specific for deubiquitinating enzymes (DUBs), was synthesiz ed as an active site directed probe that irreversibly modifies a subset of Ub C - terminal hydrolases (UCHs) and Ub specific processing proteases (UBPs), is provided [125I]-UbVS modifies 6 of the 17 known and putative yeast deubiquitinating enzymes, namely Yuh1p, Ubp1p, Ubp2p, Ubp6p, Ubp12p and Ubp15p. In mammalian cells, a greater number of polypeptides is labeled, mos t of which are DUBs. An additional DUB that associates with the mammalian 26S proteasome, novel protein USP14, a mammalian homolog of yeast Ubp6p that is bound to the proteasome, is provided.
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申请公布号 |
CA2483494(A1) |
申请公布日期 |
2003.11.06 |
申请号 |
CA20032483494 |
申请日期 |
2003.04.25 |
申请人 |
EMORY UNIVERSITY;PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
KOLLI, NAGAMALLESWARI;GALARDY, PAUL;PLOEGH, HIDDE L.;GAN-ERDENE, TUDEVIN;BORODOVSKY, ANNA;OVAA, HUIB;KESSLER, BENEDIKT;HEMELAAR, JORIS;WILKINSON, KEITH D. |
分类号 |
C07K14/00;C12N9/64;(IPC1-7):C12N15/62;C07K19/00;C07K14/47;G01N33/53 |
主分类号 |
C07K14/00 |
代理机构 |
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地址 |
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