Detection of genetic sequences using a bipartite probe

摘要

The present invention concerns a method to improve detection or quantification of a genetic sequence in a genetic sample using a bipartite probe. A bipartite probe is made of a nucleic acid binding sequence capable of hybridizing a target genetic sequence and a binding probe sequence capable of hybridizing to a detectable amplification molecule through a nucleic acid sequence capable of hybridizing to the binding probe sequence of the bipartite probe. The amplification molecule 6 can be a dendrimer that includes a label in its core and/or on any of its arms. Moreover, a secondary signal generation molecule 11 may also be added to the mixture to further increase signal. Similarly, tertiary 20 and quaternary 30 etc. amplification molecules may successively be added to yield increase signal.