METHODS FOR EVALUATION OF IN VITRO AMINOACYL TRNA PRODUCTION USING MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY

摘要

The present invention relates to a new for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Specifically, the present invention provides a new method for evaluating the aminoacylation state of tRNAs, which are useful for the site-directed muta genesis of both sidechain and backbone protein structutres. Further, the present invention useful for the generation of novel peptides,polypepides, and protein that may be used in drug design and screening, and in the design of small molecule agonists or antagonists for receptors, enzymes and other protein and molecules involed in various disease states. Additionally, the present invention is useful in the field of tRNA aminoacylation, particularly in the design of synthetases.