发明名称 |
METHODS FOR SEARCHING POLYNUCLEOTIDE PROBE TARGETS IN DATABASES |
摘要 |
Methods for determining the binding affinity of a probe to a target or targets in a polynucleotide composite using an automated system. Such methods determine relative binding sites based on thermodynamic principles, using a thermodynamic alphabet and a thermodynamic scoring matrix, with appropriate computer software, such as BLASTP. The invention also provides methods for designing polynucleotide probes to be used in hybridization assays, and minimizing the occurrence of crosshybridization. |
申请公布号 |
WO03070964(A3) |
申请公布日期 |
2003.11.06 |
申请号 |
WO2003US04354 |
申请日期 |
2003.02.14 |
申请人 |
APPLERA CORPORATION |
发明人 |
SCAFE, CHARLES, R.;PEYRET, NICOLAS;DE LA VEGA, FRANCISCO, M.;KOEHLER, RYAN, T.;SPIER, EUGENE, G. |
分类号 |
C12Q;C12Q1/68;G01N33/48;G01N33/50;G06F19/22 |
主分类号 |
C12Q |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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