FORMATION AND USE OF SITE SPECIFIC NUCLEIC ACID COUPLED BINDING POLYPEPTIDES

摘要

The present invention provides methods of covalently attaching a nucleic acid to a specific site on a binding polypeptide non-enzymatically. The methods produce large quantities of site specific nucleic acid coupled binding polypeptides. The site specific nucleic acid coupled binding polypeptides are used in accurate and sensitive methods for detecting and quantifying target analytes. Thus, the present invention provides economical and facile methods of making site specific nucleic acid coupled binding polypeptides for use in detection of target analytes.