Process for partitioning of proteins using hydrophobin proteins fused to the cell or protein of choice

摘要

The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases. The process comprises the steps of a) in order to obtain a fusion protein or cell, combining a protein or a cell of interest to a targeting protein selected from the group consisting of hydrophobins, hydrophobin-like proteins and parts thereof and b) subjecting said fusion protein or cell carrying the targeting protein to an ATPS separation.