| 摘要 |
A method for identifying a segregating, index phenotype- modifying single point mutation in a non-human animal, wherein the single point mutation occurs at a modifier locus and the index phenotype is conferred by an index locus, comprises the steps of: Outcrossing at least one male animal of a non-human founder inbred strain, the male animal carrying random point mutations relative to a would type animal of the founder inbred strain, to at least one female animal of a non-human index inbred strain. The female animal carrying a congenic dominant allele at the index locus, displaying the index phenotype and being genetically distinguishable from the founder inbred strain. To produce F1 progeny, a portion of which carry both of the dominant allele at the index locus and at least one random point mutation; Identifying one or more F1 individuals displaying an outlaying phenotype relative to the index phenotype displayed by the female animal of the index inbred strain, thereby indicating that at least one F1 individual possesses an index phenotype-modifying mutation; Backcrossing gametes from male F1 progeny to at least one female of the index inbred strain with or without the index allele to produce N2 backcross progeny, wherein at least one of the N2 backcross progeny that carry the dominant allele also exhibit the outlaying phenotype and verifying that the outlaying phenotype is caused by a segregating single point mutation. Preferably the animals are a mice. |
