Delta-5 acyl-CoA desaturase and fatty acyl-CoA elongase and subsequent recombinant methods to produce seed oil comprising destaurated fatty acid

摘要

An isolated polynucleotide comprises a nucleotide sequence that encodes a polypeptide having delta-5-acyl-CoA desaturase activity. The amino acid sequence of the polypeptide has at least 80% sequence identity with the sequence of SEQ ID NO:2. A vector and a non-human cell can comprise the polynucleotide, preferably the cell is a plant cell. The non-human cell can be used in methods to alter the level of expression of an enzyme involved in lipid biosynthesis, to produce a desaturated fatty acid comprising a double bond in the delta-5 position, to produce seed oil comprising a desaturated fatty acid wherein the fatty acid comprises a double bond in the delta- 5 position, to reducing the level of 16 carbon fatty acids in a cell and in a method of increasing the level of 20 carbon fatty acids in a cell.