| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for detecting a target nucleic acid, capable of rapidly and simply detecting presence of the target nucleic acid in a sample to be examined, compared with a conventional hybridization method and to provide a test piece for detection of the target nucleic acid suitable therefor and a kit for detection of the target nucleic acid. <P>SOLUTION: The method for detecting the target nucleic acid by using a detection probe and a capturing probe which bind to the target nucleic acid, but have no binding property to each other comprises (a) a step for mixing a labeled complex having the detection probe and a signal reporter with a sample to be examined, (b) a step for introducing the mixture obtained by the step (a) onto a water-absorbing substrate having a stationary phase obtained by immobilizing the capturing probe through bond of a ligand to a ligand- binding compound, (c) a step for developing the mixture on the water-absorbing substrate under stringent conditions and (d) a step for detecting a signal from the signal reporter in the stationary phase. The test piece for detection of the target nucleic acid is composed of the water-absorbing substrate. The kit for detection of the target nucleic acid comprises the labeled complex and the test piece. <P>COPYRIGHT: (C)2004,JPO |
