发明名称 |
METHODS OF GENERATING HUMAN CARDIAC CELLS AND TISSUES AND USES THEREOF |
摘要 |
A method of generating cells predominantly displaying at least one characteristic associated with a cardiac phenotype is disclosed. The method comprises (a) partially dispersing a confluent cultured population of human stem cells, thereby generating a cell population including cell aggregates; (b) subjecting said cell aggregates to culturing conditions suitable for generating embryoid bodies; (c) subjecting said embryoid bodies to culturing conditions suitable for inducing cardiac lineage differentiation in at least a portion of the cells of said embryoid bodies, said culturing conditions suitable for inducing cardiac lineage differentiation including adherence of said embryoid bodies to a surface, and culture medium supplemented with serum, thereby generating cells predominantly displaying at least one characteristic associated with a cardiac phenotype. |
申请公布号 |
WO03008535(A3) |
申请公布日期 |
2003.10.23 |
申请号 |
WO2002IL00606 |
申请日期 |
2002.07.21 |
申请人 |
TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.;GEPSTEIN, LIOR;KEHAT, IZHAK;ITSKOVITZ-ELDOR, JOSEPH;AMIT, MICHAL |
发明人 |
GEPSTEIN, LIOR;KEHAT, IZHAK;ITSKOVITZ-ELDOR, JOSEPH;AMIT, MICHAL |
分类号 |
A61K35/12;C12N5/02;C12N5/077;G01N33/50 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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地址 |
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