COLON TUMOR SPECIFIC BINDING PEPTIDES

摘要

Phage display was used to screen peptide libraries that distinguish between well-differentiated (HCT116) and poorly-differentiated colon carcinoma cells (HT29). The screening protocol used selection and subtraction on intact, viable cells, resulting in phage libraries exhibiting high binding selectivi ty for the poorly-differentiated HT29 cells. A nine amino acid, disulfide- constrained peptide (RPM) was identified that selectively bound and was internalized into colon cancer cells. The peptide may be used to detect colo n cancer cells and also may be used to selectively deliver therapeutic agents to the cells.