REPRODUCTION OF RIBONUCLEIC ACIDS

摘要

The invention relates to a method for reproducing ribonucleic acids. According to said method: (a) a DNA single strand is created from an RNA by reverse transcription using a single-strand primer, an RNA-dependent DNA polymerase and deoxyribonucleotide monomers; (b) the RNA is removed; (C) a DNA double strand is produced using a single-strand primer, which contains the sequence of a promoter, a DNA polymerase and deoxyribonucleotide monomers; (d) the double strand is split into single strands; (e) DNA double strands are created from the single strands created in (d), using a single-strand primer, which contains the sequence of a promoter, a DNA polymerase and deoxyribonucleotide monomers; (f) a plurality of RNA single strands is created using an RNA polymerase and ribonucleotide monomers. The invention also relates to kits comprising the components that are required for carrying out the inventive method.