The present invention provides methods for producing a nucleic acid fingerprint as well as methods for detecting sequence polymorphisms between one or more genomes. The methods involve: (a) fragmenting a nucleic acid into nucleic acid fragments with ends that are compatible to ligation with at least one adapter, (b) performing a ligation reaction between the compatible ends of the nucleic acid fragments and at least one adapter, (c) amplifying the nucleic acid fragments by using at least one amplification primer, and (d) generating a nucleic acid fingerprint from the amplified fragments. A method according to the present invention permits high-resolution fingerprinting while maintaining stringency in a PCR reaction. In another aspect, the invention also provides a kit for preparing nucleic acid fingerprints according to methods of the invention.
申请公布号
WO03087409(A1)
申请公布日期
2003.10.23
申请号
WO2003NL00253
申请日期
2003.04.04
申请人
ID-LELYSTAD, INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID B.V.;AGBO, EDWIN, CHUKWURA;TE PAS, MARINUS, FREDERICK, WILLEM;SMITS, MARINUS, ADRIANUS