METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY

摘要

A method of measuring human CYP3A inducibility at the administration of a test drug characterized by comprising, to a non-human animal to which the test drug has been administered or cultured human cells having been cultured in a medium containing the test drug, transferring (A) a virus constructed by implanting a detectable reporter gene and at least 3 sites of regions capable of binding to human PXR in the non-translation region of human CYP3A gene into an adenovirus vector, and (B) a virus constructed by implanting human PXR gene into an adenovirus vector, and then measuring the expression dose of the reporter gene in the non-human animal or the cultured human cells. According to this method, the human CYP3A inducibility at the administration of a test drug to humans can be conveniently and accurately evaluated, which makes it possible to accurately evaluate the efficacy of the test drug, the expression of its side effect, the disappearance of the drug effect, etc.